Abstract Detail

Recent Topics Posters

Miller, Allison [1], Callen, Steven [1].

Comparative transcriptome and ionome analysis in two closely related North American grapevines (Vitis riparia and V. rupestris) in a common garden.

It is well known that plant populations are adapted to local environmental conditions, and that there is a genetic basis to phenotypic variation.  An expanding area of research investigates environmental influences on phenotypes, and the mechanisms underlying how individual genotypes respond to seasonal and inter-annual climatic heterogeneity.  How does the range of phenotypic variation differ among genotypes and between species, across geography and over time?  In this study, we characterized variation in gene expression and ion concentration in leaves of Vitis riparia and V. rupestris, two closely related North American grapevines that are differentiated morphologically, genetically, and with respect to the climatic variables that characterize their natural distributions.  For both V. riparia and V. rupestris, we planted three clones each of three genotypes in a common garden at the Missouri Botanical Garden.  Leaf tissue was harvested from each individual at 12:30pm on 07/10/2013 and 08/08/2013 for RNA-seq and high throughput ionomic analysis.  Approximately 85% of transcript sequences mapped to the Vitis vinifera 12x reference genome.  Transcripts with significantly higher levels of abundance were identified in V. riparia relative to V. rupestris, and in V. rupestris relative to V. riparia at each time.  For the ionome analysis, 20 ions were quantified in leaf samples, and ions that differed significantly among species, among genotypes, and among time points for each genotype were identified.  These data point to specific transcripts that are consistently differentially expressed among species, for all genotypes sampled, and across both time points, as well as transcripts that differ on one day but not both days.  In addition, they suggest the two species differ in their ability to concentrate ions in their leaves under common conditions.  Future work will expand on these results by exploring DNA-level differences in differentially expressed transcripts and their adaptive significance.

Related Links:
Miller Lab Website

1 - Saint Louis University, Biology, 3507 Laclede Avenue, Saint Louis, MO, 63130, USA

Keywords:
none specified

Presentation Type: Recent Topics Poster
Session: P
Location: /
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PRT032
Abstract ID:1270
Candidate for Awards:None