Abstract Detail

Physiological Section

Umebese , Caroline Emosioke [1], Azeez, Toheeb Adisa [2], Adekoya, Khalid Olajide [3].

Role of Ethylenediamine Tetraacetic Acid and Salicylic Acid in Alleviating Cytogenetic Toxicity of Copper in Roots of Allium cepa (L.).

The ameliorative impact of salicylic acid (SA) and ethylene diamine tetraacetic acid (EDTA) on cytogenetic and growth changes induced in Allium cepa by toxic concentration of Cu was investigated. The onion bulbs were treated with solutions prepared from different combinations of these compounds: Cu (100.79 mmol l^-1), Cu + EDTA (1273.75 mmol l^-1), Cu + SA (1000.00 mmol l^-1), Cu + EDTA + SA and the control for 5 days. It was observed that Cu toxicity inhibited root length and root biomass by 43% and 76% respectively, and induced 71% decrease in mitotic index. Chromosomal aberrations such as anaphase bridges, stickiness and vagrant were also induced in treated roots The damaging effect of Cu on the mitotic index was reduced to 16% by EDTA and 34% by SA.. Though SA was not as effective as EDTA in protecting root length and biomass, it presented some degree of ameliorative effects on the sticky chromosomes. However, the impacts of EDTA seem to be hindered by the presence of SA. In all treatments the absorbance ratio (A_260/280) of DNA remained at 1.8-2.0, indicating that the quality of DNA was not significantly affected at the tested concentration of Cu. Chelating Cu with EDTA protected root growth and cytotoxic effects while SA corrected genotoxic effects caused by Cu toxicity.

1 - University Of Lagos, Department Of Botany, Akoka-Yaba, Lagos, Nigeria, Lagos State, N/A, 23401, Nigeria
2 - University Of Lagos, Department Of Botany, Akoka-Yaba, Lagos, Lagos, N/A, 23401, Nigeria
3 - University Of Lagos, Department Of Cell Biology and Genetics, Akoka-Yaba, Lagos, Nigeria, Lagos, N/A, 23401, Nigeria

Keywords:
Allium cepa
copper
EDTA
salicylic acid
aberrations
DNA.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PPS006
Abstract ID:70
Candidate for Awards:Physiological Section Best poster presentation