Abstract Detail

Phylogenomics

Henriquez , Claudia L [1], Arias, Tatiana [2], Pires, J. Chris [3], Croat, Thomas B. [4], Schaal, Barbara A. [5].

Phylogenomics of the plant family Araceae.

The biogeography, chromosome number evolution, and evolutionary history of the plant family Araceae have become much clearer in recent years (Cabrera et al., 2008, Chartier, 2011, Cusimano et al., 2011, Cusimano et al., 2012, Nauheimer et al., 2012).  However, phylogenetic ambiguity near the root of the tree precludes answering questions about the early evolution of the family.  We use Illumina sequencing technology and reference based assembly to resolve the remaining questions in the deep phylogeny of Araceae. We sampled 32 genera and obtained 7 from GenBank (including an outgroup), representing 42 of 44 major clades described in Cusimano et al. (2011).  A subsequent phylogenomic analysis based on mitochondrial data was performed to test congruence between plastid and mitochondrial data for phylogenetic inference.  Plastid sequences produced strongly supported phylogenies.  In contrast, mitochondrial phylogenies were weakly supported and incongruent with chloroplast data (Templeton test, p=<0.0001), although several smaller clades were recovered.  New strongly-supported clades seen in this study are:  (1) Anubias and Montrichardia, excluding Calla, form a clade that is sister to the Zantedeschia clade; (2) the South African genus Zantedeschia is sister to the Old World Anchomanes clade; and (3) within the Zantedeschia clade, Philodenron (Homalomena clade) is sister to the rest.  Despite strong statistical support for most nodes in the chloroplast phylogenies, placement of the bisexually-flowered genus Calla remains unclear, as does the placement of Schismatoglottis based on complete chloroplast sequences.

1 - Washington University in St. Louis, Evolution, Ecology & Population Biology, One Brookings Drive, Campus Box 1137, St. Louis, MO, 63130, USA
2 - The University Of Hong Kong, School Of Biological Sciences, Potfulam Rd., Hong Kong, N/A, Hong Kong SAR
3 - University Of Missouri, 371 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211-7310, USA
4 - Missouri Botanical Garden, 4344 Shaw Blvd., St. Louis, MO, 63110, USA
5 - Washington University, Department Of Biology, CAMPUS BOX 1137, 1 Brookings Drive, St. Louis, MO, 63130-4899, USA

Keywords:
Araceae
next generation sequencing
clade
Phylogeny.

Presentation Type: Oral Paper:Papers for Topics
Session: 43
Location: Evergreen/Grove
Date: Wednesday, July 30th, 2014
Time: 4:15 PM
Number: 43010
Abstract ID:705
Candidate for Awards:Margaret Menzel Award