Abstract Detail

Genetics Section

Klein, Laura [1], Pap, Daniel [2], Kovacs, Laszlo [2], Hyma, Katie [3], Londo, Jason [4], Miller, Allison [5].

Genotyping-By-Sequencing reveals distinct genomic features of North American Vitis species used for grape rootstock cultivation.

Plant breeders commonly utilize hybridization to introduce genetic variation into crops. This technique is often employed with grape (Vitis; Vitaceae) rootstocks, which are grafted to shoots to provide beneficial traits, such as increased biotic and abiotic stress resistance. Not surprisingly, evidence of hybridization between Vitis species exists in the wild, yet relatively little is known about its impact on population genetic variation. The goal of this study is to assess the degree of naturally occurring hybridization between two economically important North American Vitis species (V. riparia and V. rupestris), both bred for their abiotic and biotic stress tolerance. Using genotyping-by-sequencing data (GBS), we are investigating genomic variation and potential gene flow between wild populations of V. riparia and V. rupestris occurring in both overlapping and nonoverlapping distributions (i.e., sympatric and allopatric regions, respectively). By mapping single nucleotide polymorphisms (SNPs) collected from wild populations of V. riparia and V. rupestris to the GBS pipeline developed for the cultivated grape, Vitis vinifera cv. Pinot Noir, several thousand SNPs per individual are being analyzed to test the hypotheses that unique genomic features exist within populations of Vitis species that are in allopatry, and interspecific hybridization is occurring among different species in sympatric populations. Preliminary results using individuals of V. riparia and V. rupestris suggest GBS is a useful tool for identifying distinct genomic features between these two species.

1 - Saint Louis University, Department Of Botany, 3507 Laclede Ave, Saint Louis, MO, 63108, USA
2 - Missouri State University
3 - Cornell University
4 - United States Department of Agriculture , Agricultural Research Service - Grape Research Genetics Unit
5 - Saint Louis University

Keywords:
Genotyping by Sequencing
population genomics
Vitis
grape
hybridization .

Presentation Type: Poster:Posters for Sections
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PGN004
Abstract ID:856
Candidate for Awards:Genetics Section Poster Award