Abstract Detail

Systematics Section/ASPT

ONUMINYA, TEMITOPE [1], Ogundipe, Oluwatoyin [1].

DNA Conservation and Barcoding of African Sapindaceae.

Sapindaceae Jussieu is a family of flowering plants in the order Sapindales. They exist as trees and shrubs, and tendril-bearing vines with about 140-150 genera and 1400-2000 species worldwide. They are economically, medicinally and aesthetically useful. This research aimed at exploring the diversity of Sapindaceae in western and central africa with particular emphasis on preservation of the plant samples as well as generation of DNA barcodes with a view to sharing the DNA barcode sequence in a public database. This was achieved following Doyle and Doyle (1987), Soltis and Soltis (1998b), Savolainen et al. (2000a), Savolainen et al. (2000b), Harrington et al. (2005), Buerki et al. (2009), Ogundipe and Chase (2009), Powell et al., 2009 and Ronquist et al. (2009). Extracted DNA samples (119) were deposited at the DNA Bank of the Royal Botanic Gardens, Kew, Richmond and voucher specmens were deposited at the University of Lagos Herbarium, Lagos, Nigeria. Silica gel dried specimens’ yielded good quality DNA unlike the old dried herbarium leaf samples. DNA samples were sent to International Barcode of Life (IBOL) Centre in Guelph, Canada for analysis of the barcode region and sixty-nine (69) DNA barcodes were generated. Barcode data which was hinged on matK and rbcL sequence data have been deposited at the Barcode of Life database (BOLD) website and GenBank for public use. This research can be seen as a basis for further taxonomic research on the family Sapindaceae especially in Africa.

1 - University of Lagos, Department of Botany, Akoka, Yaba, Lagos, Lagos, 23401, Nigeria

Keywords:
Africa
Bio-conservation
Sapindaceae.

Presentation Type: Oral Paper:Papers for Sections
Session: 17
Location: Payette/Boise Centre
Date: Tuesday, July 29th, 2014
Time: 8:00 AM
Number: 17001
Abstract ID:106
Candidate for Awards:George R. Cooley Award