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Abstract Detail

Recent Topics Posters

Benson, Christopher [1], Ballard, Harvey [2], Thammina, Chandra [3], Olsen, Richard [3], Pooler, Margaret [3].

Microsatellite markers developed to characterize winter hazel (Corylopsis) species.

Abstract. Corylopsis is a deciduous ornamental shrub in the witch hazel family (Hamamelidaceae) that is known primarily for its bright yellow early spring blooms and attractive fall foliage.  The genus contains approximately 25 species that occur primarily in temperate areas in eastern Asia.  Although morphological characteristics are well documented, there are questions about species identity and ploidy in the genus.  We created microsatellite (SSR) genetic markers using shotgun genome sequencing.  Of the 307 primers that were deemed to be optimal, 30 were selected to test  on diverse Corylopsis accessions. We found that 12 of the primers amplified a DNA product, and that 5 of these primers showed alleles in the appropriate size range  for each of the tested accessions when analyzed on an ABI3730 genetic analyzer.  We plan to use these primers to examine genetic diversity and identity of 115 Corylopsis accessions representing diverse species and cultivars, including C. glabrescens, C. glandulifera, C. gotoana, C. pauciflora, C. sinensis, C. spicata, C. veitchiana, and C. willmottiae. The microsatellite markers developed for this study could aid in taxonomic research of Corylopsis and help to guide breeding and collection decisions in our program.

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1 - Ohio University, Department of Environmental & Plant Biology, Athens, OK, 45701-2979, USA
2 - Ohio University, ENVIR & PLANT BIOLOGY-PORTER H, 315 Porter Hall, Athens, OH, 45701-2979, USA
3 - USDA-ARS U.S. National Arboretum, 10300 Baltimore Ave., Beltsville, MD, 20705, USA

none specified

Presentation Type: Recent Topics Poster
Session: P
Location: /
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PRT034
Abstract ID:1272
Candidate for Awards:None

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