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Abstract Detail

Pteridological Section/AFS

WOLF , PAUL G [1], Der, Joshua [2], Li, Fay-Wei [3], Rothfels, Carl J [4], Gitzendanner, Mathew A [5], Visger, Clayton J [6], Marchant, D. Blaine [6], Soltis, Douglas E. [7], SOLTIS, PAMELA S. [8], Pryer, Kathleen M [9].

An exploration of fern genome space.

Ferns are one of the few remaining major clades of land plants for which a complete genome sequence is lacking. Knowledge of genome space in ferns will enable broad-scale comparative analyses of land plant genes and genomes, provide insights into genome evolution across green plants, and shed light on genetic and genomic features that characterize ferns, such as their high chromosome numbers and large genome sizes. As part of an initial exploration into fern genome space, we used a whole genome shotgun sequencing approach to obtain low-density coverage (1X to 2X) for six fern species from the Polypodiales (Ceratopteris, Pteridium, Polypodium, Cystopteris), Cyatheales (Plagiogyria), and Gleicheniales (Dipteris). We explore these data to characterize the proportion of the nuclear genome represented by protein-coding genes and repetitive sequences (including transposons, rDNA, and satellite elements), as well as to extract chloroplast and mitochondrial genome sequences. Such initial sweeps of fern genomes can provide information useful for selecting a promising candidate fern species for whole genome sequencing. 

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1 - Utah State University, Department Of Biology, 5305 OLD MAIN HILL, Logan, UT, 84322-5305, USA
2 - Penn State University, Department Of Biology, 201 Life Sciences Building, University Park, PA, 16802, USA
3 - Duke University, Biology, Durham, NC, 27707-0338, USA
4 - University Of British Columbia, Department Of Biology, #4200-6270 University Blvd., Vancouver, BC, V6T 1Z4, Canada
5 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, GAINSVILLE, FL, 32611-7800, USA
6 - University of Florida, Florida Museum of Natural History and Department of Biology, Gainesville, FL, 32611, United States
7 - University of Florida, Florida Museum of Natural History, Gainesville, FL, 32611
8 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA, 352/273-1964
9 - Duke University, Science Drive, Durham, NC, 27708-0338, USA

organelle genomes
mitochondrial genome
plastid genome.

Presentation Type: Oral Paper:Papers for Sections
Session: 7
Location: Pines North/Boise Centre
Date: Monday, July 28th, 2014
Time: 11:45 AM
Number: 7013
Abstract ID:145
Candidate for Awards:None

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