Abstract Detail

Systematics Section/ASPT

BOMBARELY, AURELIANO [1], SHERMAN-BROYLES, SUE [1], COATE, JEREMY E [2], DOYLE , JEFF J [3].

Dissecting relationships in the Glycine (Leguminosae) polyploid complex with genome-wide data.

The Glycine polyploid complex, as currently understood, comprises a group of nine polyploid taxa. Eight of these are allopolyploids formed from crosses involving eight diploid taxa; most of the polyploids appear to have originated multiple times. Taxonomy of the group is complicated, with four diploid and five tetraploid taxa all classified as G. tomentella Hayata. Hypotheses of genome origins are based on a foundation of artificial hybridization studies, supported by data from pre-genomic era molecular systematic studies. To understand the origins and relationships of the allopolyploids at the level of detail needed to exploit them more effectively as a model for studying polyploid evolution, we wish to 1) corroborate hypotheses of origins using markers spanning the entire genome; and 2) use such markers to identify population structure in the polyploids and in their diploid progenitors. The latter is of interest in addressing the question of whether an allopolyploid that formed multiple times from different genotypes of the same diploid genomes exists as a single evolutionary lineage united by gene flow, or whether it comprises several distinct lineages, potentially traceable to subpopulations within its progenitors. Transcriptome data have been used to address the first question for a limited number of species and accessions, and show that all allopolyploid plants thus far studied are fixed hybrids, combining markers from both hypothesized parents throughout their entire genomes. The much wider sampling needed to address the second question will require more affordable approaches, such as genotyping by sequencing (GBS), which appears to be effective for assaying variation in both the nuclear and organellar genomes.

1 - Cornell University, Plant Biology, 412 Mann Library Building, Ithaca, NY, 14853-4301, USA
2 - Reed College, Plant Biology, 3203 SE Woodstock Blvd., Portland, OR, 97202, USA
3 - Cornell University, 412 Mann Library Building, ITHACA, NY, 14853-4301, USA

Keywords:
allopolyploidy
Glycine
whole genome duplication
genome-wide variation.

Presentation Type: Oral Paper:Papers for Sections
Session: 18
Location: Salmon/Boise Centre
Date: Tuesday, July 29th, 2014
Time: 8:45 AM
Number: 18004
Abstract ID:232
Candidate for Awards:None