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Abstract Detail

Conference Wide

Liston, Aaron [1].

Introduction to Next Generation Sequencing.

Next generation sequencing (NGS) describes technologies that produce billions of base pairs of sequence data per experiment. The low cost of these technologies (currently 10 Mbp / dollar) allows an increasing number of biologists to incorporate genome-scale sequencing into their research. The goal of this workshop is to introduce participants to the essentials of NGS, so that they can develop and initiate their own projects in phylogenetics, population genetics, and gene expression using this technology. In the morning, we will describe the available technologies, applications, and protocols for sample preparation and sequencing. We will also describe options for computational infrastructure and analytical pipelines. In the afternoon, participants will then have an opportunity to work with data from the Illumina platform using both commercial software (Geneious) and the Linux operating system with open source scripts.  Topics covered will include plastome sequence assembly and probe design for nuclear gene target enrichment (Hyb-Seq).  A laptop computer is required. Fees will cover internet access, extension cords for laptops, a coffee break and lunch.

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1 - Oregon State University, Department Of Botany & Plant Pathology, 2082 Cordley Hall, Corvallis, OR, 97331-2902, USA, 541/737-5301

none specified

Presentation Type: Workshop
Session: WS02
Location: Ivy/Grove
Date: Sunday, July 27th, 2014
Time: 8:00 AM
Number: WS02001
Abstract ID:41
Candidate for Awards:None

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