Genomics / Proteomics
COATE, JEREMY E , Song, Michael , DOYLE , JEFF J .
Polyploidy and transcriptome size: does genome doubling cause transcriptome doubling?
Genome duplication and subsequent downsizing are relatively well characterized facets of polyploid evolution, but little is known about the effects of polyploidy and diploidization on transcriptome size (the total number of transcripts per cell). We are quantifying transcriptome size in synthetic and recently formed natural polyploids in order to determine the immediate effects of genome duplication as well as subsequent evolution on total transcription. The leaf transcriptomes of two closely related and recently formed allopolyploid relatives of soybean are significantly larger than those of their diploid progenitors, but significantly less than their sum. In one allopolyploid examined to date (G. dolichocarpa), the root transcriptome is ca. 4-fold larger than the leaf transcriptome. In contrast, transcriptomes from a given tissue are of comparable size across four diploid species and across two tetraploid species, suggesting that total transcription is under tight control within a given tissue and ploidy level. We are using estimates of transcriptome size to infer individual gene dosage responses (changes in transcript number due to changes in gene copy number), and to determine if these co-vary with structural and/or functional properties of the encoded proteins, thereby rigorously testing dosage-based theories of gene and genome evolution.
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1 - Reed College, Plant Biology, 3203 SE Woodstock Blvd., Portland, OR, 97202, USA
2 - Reed College, Biology, 3203 SE Woodstock Blvd, Portland, OREGON, 97202, United States
3 - Cornell University, 412 Mann Library Building, ITHACA, NY, 14853-4301, USA
Presentation Type: Oral Paper:Papers for Topics
Location: Pines South/Boise Centre
Date: Wednesday, July 30th, 2014
Time: 9:15 AM
Candidate for Awards:Margaret Menzel Award