Abstract Detail

Molecular Ecology and Evolution

Wysocki, William P. [1], Clark, Lynn G. [2], Kelchner, Scot A. [3], Attigala, Lakshmi [4], Ruiz-Sanchez, Eduardo [5], Duvall, Melvin R. [6].

A full-plastome phylogenomic study on the tropical bamboo lineages.

The Bambusoideae (Poaceae) are comprised of three distinct and well-supported lineages: tropical woody bamboos (Bambuseae), temperate woody bamboos (Arundinarieae) and herbaceous bamboos (Olyreae).  Phylogenetic studies using chloroplast markers have generally supported a sister relationship between Bambuseae and Olyreae. This suggests either at least two origins of the woody character in this subfamily or a loss of the woody habit in Olyreae. Here a full chloroplast genome (plastome) phylogenomic study is presented using the coding and noncoding regions of 22 complete bambusoid plastomes from the hypothesized Olyreae-Bambuseae clade and two plastomes from the Arundinarieae. A nuclear marker, granule-bound starch synthase I (GBSSI), was also used to generate a phylogeny with somewhat different taxonomic sampling.  Trees generated using plastome sequences support the previously acknowledged monophyletic relationship between Bambuseae and Olyreae while those generated with nuclear sequences support a monophyletic origin of the woody Bambusoideae.  A parametric bootstrap analysis failed to find evidence of long-branch attraction on the topology of Bambusoideae from nuclear gene trees. Incongruence between nuclear and plastome trees can indicate hybridization.  In this case, a hypothetical hybridization event causing the observed incongruent topologies would need to have occurred early in the diversification of Bambusoideae.  Our hypothesis could be confirmed with transcriptomic techniques to increase sampling among nuclear orthologues and investigate the molecular genetics underlying the development of woody tissues.

1 - Northern Illinois University, Biological Sciences, 1425 W. Lincoln Hwy, DeKalb, IL, 60115, USA
2 - Iowa State University, Department Of Ecology, Evolution, And Organismal Biology, 251 Bessey Hall, Ames, IA, 50011-1020, USA
3 - Idaho State University, Department Of Biological Sciences, 921 South 8th Ave., Stop 8007, Pocatello, ID, 83209-8007, USA
4 - Iowa State University, Department of Ecology Evolution and Organismal Biology, 251 Bessey Hall, Ames, IA, 50011, USA
5 - INECOL, Centro Regional Del Baj’o, L‡zaro C‡rdenas 253, P‡tzcuaro, Michoac‡n, 61600, Mexico
6 - NORTHERN ILLINOIS UNIVERSITY, Department Of Biological Sciences, 1425 W Lincoln Hwy, DEKALB, IL, 60115-2861, USA

Keywords:
phylogenomics
Poaceae
Bambusoideae
GBSSI
plastome.

Presentation Type: Oral Paper:Papers for Topics
Session: 11
Location: Firs North/Boise Centre
Date: Monday, July 28th, 2014
Time: 1:45 PM
Number: 11002
Abstract ID:439
Candidate for Awards:None