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Abstract Detail

Genomics / Proteomics

Sinn, Brandon T. [1], Freudenstein, John [2].

Organellar Genome Evolution in Asarum (Aristolochiaceae).

The evolution of organellar genome content, size, and arrangement is currently fraught with activity due to the use of large scale massively parallel sequencing platforms that can easily sequence them in their entirety in a single run. The magnoliids contain approximately 5% of angiosperm species, yet fully sequenced chloroplast genomes are available from only three genera (Drimys, Liriodendron, and Piper) and the mitochondrial genome from only Liriodendron. We are currently assembling the mitochondrial and chloroplast genomes of Asarum canadense and Asarum minus, using a combination of Illumina MiSeq 300 bp and HiSeq 100bp paired-end sequencing, in Geneious. Analysis of 67 chloroplast coding regions strongly supports Asarum as a member of the Piperales with Piper as its sister in a highly derived clade within the magnoliids, a long-supported relationship. Transfer of chloroplast sequence to the mitochondrion has occurred in both species, and these mitochondrial genomes bear little semblance to the primitive mitochondrial genome of Liriodendron in regards to size and synteny, yet share all tRNAs. We observe explosive expansion of homopolymer repeats and intergenic microsatellite sites in the chloroplasts of both species, some over 15 and 150 base pairs in length, respectively. We posit that microsatellite regions may have played a role in horizontal transfer of gene regions from the chloroplast to the mitochondrion. Twenty percent of  previously identified RNA editing sites in the Liriodendron mitochondrion have been lost in Asarum, suggesting that the same genomic trends have impacted the magnoliids as eudicot and monocot lineages.

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Related Links:
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1 - Ohio State University, Evolution, Ecology and Organismal Biology, 1315 Kinnear Rd, Columbus, OH, 43212, USA
2 - Ohio State University, MUSEUM OF BIOLOGICAL DIVERSITY, 1315 KINNEAR RD, COLUMBUS, OH, 43212-1157, USA

plastid genome
mitochondrial genome.

Presentation Type: Oral Paper:Papers for Topics
Session: 35
Location: Pines South/Boise Centre
Date: Wednesday, July 30th, 2014
Time: 11:30 AM
Number: 35014
Abstract ID:447
Candidate for Awards:None

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