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Abstract Detail

Systematics Section/ASPT

Shipunov, Alexey [1], Keila, Aguilar [2], Hye Jee, Lee [2].

Phylogeny of plantains (Plantago L., Plantaginaceae).

Plantains (Plantago L.) are widely distributed herbs and shrubs from the asterid family Plantaginaeae. There are more than 200 species of Plantago (Rahn, 1996). Plantains exhibit the transition from the insect-pollinated to wind-pollinated flowers (Preston et al., 2011) and unusually fast evolution (Ronsted et al., 2002; Cho et al., 2004): for 5.5 million years, this group produce hundreds of species and colonized of all continents including Antarctic. Unfortunately, the most detailed phylogeny of genus includes only 23 species (Hoggard et al., 2003). We decided to sequence as many species of genus as possible. Since the fresh material is often unavailable, we use herbarium samples from U.S. and international herbarium collections. With the collaboration of International Barcode of Life Project (iBoL), we checked several phylogenetic markers (coi5, rbcL, trnlF, ITS) and found that nuclear ITS2 pseudogene allows to make trees with the best resolution on the species level. ITS2 has the relatively short length (~ 330 bp) and therefore is suitable for amplification from the partially degraded DNA extracted from herbarium samples. So far, we sequenced more than 40 species which were not yet represented in GenBank, including all North American plantains (half of them were not sequenced before). During the DNA extraction process, we found that even older samples have DNA concentration > 10 ng/microliter which should allow for the successful PCR. There is only weak relation between the amount of extracted DNA and age of collection.

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1 - Minot State University, Biology, 500 University Ave, Minot, ND, 58707, USA
2 - Minot State University, Biology, 500 University Ave W, Minot, ND, 58707, USA


Presentation Type: Poster:Posters for Sections
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PSY040
Abstract ID:456
Candidate for Awards:None

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