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Abstract Detail



Molecular Ecology and Evolution

Duvall, Melvin R. [1], Wysocki, William P. [2].

First complete chloroplast genome sequence from Joinvilleaceae (J. ascendens, Poales) shows unique and unpredicted rearrangements.

Joinvilleaceae comprise a minor component of the tropical Australo-Pacific flora.  However, together with Ecdeiocoleaceae the family is evolutionarily significant because of a close relationship to the grasses.  Joinvilleaceae were previously reported to have a chloroplast genome (plastome) with two major inverted segments when compared to those of most non-grass monocots.  This was further postulated to be an intermediate organizational condition to the more highly rearranged plastomes of grasses.  We prepared a single-end DNA library from J. ascendens that was sequenced using next-generation technology.  A plastome of 149,363 bp was assembled from 8.98 million quality-trimmed reads using a completely de novo method of in silico genome walking. The basic quadripartite structure and gene content are preserved in this plastome.  However, gene order and orientation are significantly altered as expected.  Our fully sequenced plastome precisely indicates the inversions predicted by previous workers.  However, at least two other unsuspected major inversion events encompassing the previously documented inversions and spanning tens of kilobases were also observed.  Since these events are not shared by any Poaceae, we hypothesize their occurrence after Joinvilleaceae diverged from the lineage that eventually gave rise to Poaceae.  Sanger sequencing of over 60% of the plastome of J. plicata and bioinformatics analyses of our complete plastome and inversion endpoints identified here support our de novo assembly and suggest that the earlier characterizations, although broadly correct, failed to completely characterize the numbers and locations of major inversions. Our plastome sequence of J. ascendens can serve as a standard for the sequencing of plastomes in other non-grass Poales. Whether the unique events we document in Joinvilleaceae are synapomorphic with other Poales awaits the sequencing of plastomes of Ecdeiocoleaceae or other representatives in the order.


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1 - NORTHERN ILLINOIS UNIVERSITY, Department Of Biological Sciences, 1425 W Lincoln Hwy, DEKALB, IL, 60115-2861, USA
2 - Northern Illinois University, Biological Sciences, 1425 W. Lincoln Hwy, DeKalb, IL, 60115, USA

Keywords:
Poales
Joinvilleaceae
plastome.

Presentation Type: Oral Paper:Papers for Topics
Session: 28
Location: Firs South/Boise Centre
Date: Tuesday, July 29th, 2014
Time: 1:45 PM
Number: 28002
Abstract ID:468
Candidate for Awards:None


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