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Abstract Detail



Genomics / Proteomics

Darby, Hayley [1], Demes, Kyle [2], Iles, William [1], Graham, Sean W. [1].

Next-generation plastome sequencing of the feather boa kelp, Egregia menziesii (Laminariales).

We report on the assembly of the complete plastid genome of the northeastern Pacific kelp species Egregia menziesii. This is the second fully assembled laminarian brown alga (the other is kombu, Saccharina japonica); we selected the morphologically distinct species, E. menziesii, as a trial for whole plastid genome sequencing. We recovered the genome using genome-survey sequencing and de novo assembly, bridging several gaps using PCR and Sanger sequencing. The ~125 kb genome of Egregia includes a large single copy (LSC) region, a small single copy (SSC) region, and a relatively small inverted repeat (IR). Gene order and content in Egregia is largely congruent with that in Saccharina. A previous phylogenetic study had difficulty placing E. menziesii in higher-order kelp phylogeny, weakly supporting it as the sister group of a clade of broadly distributed kelps. This may reflect an early, rapid radiation in the order around the Oligocene. Our sequence of Egregia is intended to initiate additional sampling of complete plastid sequences, for resolving these early splits in kelp phylogeny. A well-supported whole plastid genome phylogeny would provide a foundation for investigating questions about the evolution of morphology, life history traits, and habitat preference in this order of ecologically and economically important macroalgae. 


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1 - University of British Columbia, Department of Botany, 3529 - 6270 University Boulevard, Vancouver, BC, V6T 1Z4, Canada
2 - Simon Fraser University, Hakai Network, School of Resource and Environmental Management, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada

Keywords:
plastid genome
brown algae
kelp
Laminariales
Phaeophyta
Heterokontophyta.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PGP005
Abstract ID:501
Candidate for Awards:Genetics Section Poster Award


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