Create your own conference schedule! Click here for full instructions

Abstract Detail

Systematics Section/ASPT

Granados Mendoza, Carolina [1], Naumann, Julia [2], Samain, Marie-Stéphanie [3], Goetghebeur, Paul [4], De Smet, Yannick [4], WANKE, STEFAN [5].

On the Hunt for rapidly-evolving nuclear single- or low copy loci to address shallow-scale phylogenetics in Hydrangea section Cornidia.

Identifying orthologous molecular markers that potentially resolve relationships at species level and below has been a major challenge in plant phylogenetics over the past decade. Non-coding parts of nuclear low or single-copy markers are a vast and promising source that potentially provides information for shallow-scale phylogenetics. Recent low-cost transcriptome sequencing projects such as the One Thousand Plant Project (1KP) provide public access to nuclear sequence data for a number of non-model organisms. The present study takes advantage of this transcriptome data for developing rapidly-evolving nuclear, potentially orthologous single-copy markers to address shallow-scale phylogenetics. We use Hydrangea section Cornidia (Hydrangeaceae, Cornales) as study group; one of the shallowest diverged lineages within Hydrangeeae. In the past, phylogenetic relationships within this group have been particularly difficult to reconstruct due to extremely low plastid variability. Previous studies identified sets of nuclear genes that occur in single or low-copy among several angiosperm representatives. Starting from these datasets, we developed two gene search strategies: 1) genome-scale mining strategy and 2) gene sequence-based strategy. A pool of 73 putative single-copy nuclear markers was recovered from the genome-scale mining strategy and five genes from the sequence-based strategy. Here we present first results from two of these genes, the TIF3H1 gene from the genome-scale mining strategy and the SMC1 gene from the sequence-based strategy. Analyses of the concatenated nuclear markers dataset provide both resolution and support for most nodes in the phylogenetic tree. Based on our nuclear phylogenetic framework we discuss phylogenetic relationships within Hydrangea sect. Cornidia, biogeographic implications, and contribute to our knowledge of the distribution and evolution of sexual dimorphism in the group.

Log in to add this item to your schedule

1 - Río Hondo 71, Colinas Del Lago, Cuautitlán Izcalli, Estado De México, N/A, 54744, Mexico
2 - TU Dresden, Biology, Zellescher Weg 20b, Dresden, 01062, Germany
3 - Inecol, Mexico
4 - Gent University, Belgium
5 - TU Dresden, Dept. Of Biology, Institut Of Botany, Zellescher Weg 20b, Dresden, N/A, 01062, Germany

Data mining
fine-scale phylogenetics
Hydrangea section Cornidia
nuclear low or single copy genes
sexual dimorphism

Presentation Type: Oral Paper:Papers for Sections
Session: 30
Location: Payette/Boise Centre
Date: Tuesday, July 29th, 2014
Time: 3:45 PM
Number: 30008
Abstract ID:542
Candidate for Awards:None

Copyright © 2000-2013, Botanical Society of America. All rights reserved