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Abstract Detail

Systematics Section/ASPT

Kates , Heather Rose [1], SOLTIS, PAMELA S. [2], Soltis, Douglas E. [2].

A phylogenetic study of the wild and domesticated pumpkins and squashes (Cucurbita, Cucurbitaceae) based on microfluidic PCR and next generation sequencing of 48 nuclear loci.

Phylogenetics can aid the study of plant domestication by resolving sister relationships between crops and their wild relatives, thereby identifying the ancestors of cultivated plants.  Our preliminary phylogenetic study of Cucurbita (pumpkins and squashes) using five nuclear loci did not find strong support for some of the currently held domestication hypotheses and ecological divisions in the genus, demonstrating the need for increased genetic sampling.  An updated phylogenetic study of Cucurbita using 48 loci derived from introns of single-copy nuclear genes provides new insight into relationships between wild and domesticated species and into the evolution of the two ecologically distinct types of Cucurbita.  The keys to this study that allow us to address previously unresolved questions are: 1) increased taxon and population sampling compared with previous studies, and 2) the use of novel genetic loci that have great utility at the population and species levels.  The set of Cucurbita accessions analyzed includes two to three representatives of all 22 species and subspecies, covering the geographic and taxonomic range of the genus.  Our application of microfluidic PCR and next-generation sequencing for this broad range of samples allows us to resolve relationships within Cucurbita and provide new insights into its multiple domestications, while demonstrating the utility of amplicon sequencing for generating maximally useful phylogenetic data for medium-sized projects.

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1 - University of Florida, Biology, Dickinson Hall, PO Box 117800, Gainesville, Florida, 32611, United States
2 - University of Florida, Florida Museum of Natural History, PO Box 117800, Gainesville, FL, 32611, USA

target enrichment
single copy nuclear genes
next generation sequencing.

Presentation Type: Oral Paper:Papers for Sections
Session: 18
Location: Salmon/Boise Centre
Date: Tuesday, July 29th, 2014
Time: 8:00 AM
Number: 18001
Abstract ID:560
Candidate for Awards:None

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