Abstract Detail

Phylogenomics

McKain, Michael [1], Hartsock, Ryan [2], Hodge, John [3], Layton, Daniel [1], Pasquet, Rémy [4], Vela, Dilys [5], Welker, Cassiano [6], Wilson, Mark [7], Zhong, Jinshun [8], Kellogg, Elizabeth [9].

Phylogeny of Andropogoneae (Poaceae) and a new tool for automated assembly of plastomes.

We have assembled a phylogeny of the grass tribe Andropogoneae using sequences of entire chloroplast genomes.  To accelerate data analysis, we are developing a computational pipeline that produces annotated plastid genomes with relatively little input aside from raw high-throughput sequence data. The final plastome sequences are properly orientated to the general format of LSC-IRB-SSC-IRA, annotated, and imported into an online plastome database.  Sequence data can be exported as aligned or unaligned files, or can be viewed in linearized form in a graphical display comparing the newly assembled plastome to those previously published. Plastome phylogenies of Andropogoneae are well resolved and generally support previous results from nuclear genes.  Andropogoneae are monophyletic, with a strongly supported core clade containing most of the ecological dominants.  Although the awned and awnless taxa have been used in the past as informal groups, neither is monophyletic.  Arundinella and Arthraxon are successively sister to the rest of the tribe.  Although both genera are C₄, they have atypical leaf anatomy, with veins separated by isolated bundle sheath cells.  The phylogeny suggests that this leaf structure could be a transitional step toward development of full-fledged C₄ leaf anatomy.  A few differences between nuclear and chloroplast trees could be the result of ancient hybridization, although at the moment we cannot rule out sampling artifacts.

1 - Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO, 63132, USA
2 - University of Missouri-St. Louis, Math and Computer Science, One University Boulevard, St. Louis, MO, 63121, USA
3 - University Of Missouri-St. Louis, Department Of Biology, One University Blvd., St. Louis, MO, 63121, USA
4 - Icipe, P.O. Box 30772, Nairobi, Kenya
5 - Washington University, Department of Biology, St. Louis, MO, 63130, USA
6 - Universidade FEderal do Rio Grande do Sul, Programa de Pós-Graduaçao em Botánica, Av. Bento Gonçalves 9500, Porto Alegre, RS, CEP 91501, Brazil
7 - University of Missouri-St. Louis, Math and Computer Science, One University Boulevard, St. Louis, MO
8 - University of Vermont, Department of Plant Biology, Burlington, VT, 05405, USA
9 - Donald Danforth Plant Science Center, Department Of Biology, 975 North Warson Road, St. Louis, MO, 63132, USA

Keywords:
Poaceae
plastome
Panicoideae
Photosynthesis.

Presentation Type: Oral Paper:Papers for Topics
Session: 43
Location: Evergreen/Grove
Date: Wednesday, July 30th, 2014
Time: 4:00 PM
Number: 43009
Abstract ID:673
Candidate for Awards:Margaret Menzel Award