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Abstract Detail



Molecular Ecology and Evolution

Choi, Sun Ah [1], Won, Hyosig [1].

Gene phylogeny in genomic era: molecular evolution of nuclear xdh gene in Brassicaceae.

With the development and application of Next Generation Sequencing method, whole genome sequence data has become popular. Especially for Brassicaceae,WGS are already available for Arabidopsis thaliana, A. halleri, A. lyrata,  Aethionema arabicum, Brassica rapa, Camelina sativa, Capsella rubella, Eutrema halophilum, E. parvulum, E. salsugineum, Leagenworthia alabamica, Sisymbrium irio, and increasing. Still, there are much more species in Brassicaceae and application of NGS to species level phylogeny and evolution is not realistic. Here we amplified and sequenced nuclear xdh gene region of representatives of Brassicaceae, especially those naturally distributed in Korea, as a window to understand evolution of gene when genome undergoes changes such as duplication, hybridization amd allopolyploidization. For Arabidopsis thaliana, xdh gene has tandemly duplicated and diverged functionally, thus requires cloning of PCR product to separate each copy. Also many Brassicaceae species presented mulitple copies of xdh gene, probably due to tandem duplication of the gene or to remnants of ancient genome duplication or hybridization. Based on the sequence information of those xdh copies, we will reconstruct the history of xdh gene evolution.


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1 - Daegu University, Department of Biological Science, 201 Daegudaero, Jillyang-eup, Gyungsan, Gyungbuk, 712-714, Korea

Keywords:
Brassicaceae
gene sequence evolution
xdh gene
Phylogeny
Genome duplication.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PME008
Abstract ID:682
Candidate for Awards:None


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