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Abstract Detail

Genomics / Proteomics

Broe, Michael [1], Freudenstein, John [2].

Plastome reduction and rearrangement in members of the mycoheterotrophic Monotropoideae (Ericaceae).

The Monotropoideae are an herbaceous, non-photosynthetic subfamily of the Ericaceae, whose members form obligate symbioses with mycorrhizal fungi (which are in turn symbiotic with an autotrophic host). Most species are endemic to North America. We present whole plastome sequences for five closely related taxa in the Monotropoideae: Allotropa virgata, Hemitomes congestum, Monotropa hypopithys, and two accessions of Pityopus californicus. We present our findings in the context of recently published plastomes from (i) Ericaceae (including an example from the Arbutoids, sister tribe to the Monotropoids) and (ii) holoparasites from the Orobanchaceae, including Conopholis americana. Like holoparasites, mycoheterotrophs exhibit highly reduced plastomes. The gene complement of monotropoids consists of 4 rRNAS, 18 tRNAs, rps and rpl subunits (which bear a striking similarity to those retained by Conophilis), together with matK and infA. There are no NADH, rbcL, ATP synthase, photosystem I or II, or plastid-encoded polymerase (rpo) genes. There also appears to be a single IR region, resulting in a genome which, at approx 35 kb, is some 10 kb shorter than Conopholis.
Whole genome sequencing also reveals that most monotropoids have a pseudogenized copy of rps2 in the mitochondrial genome, unusual for dicots, which coamplifies (or preferentially amplifies) with cp-rps2 in Sanger sequencing using standard primers. We distinguish these two copies, and show how the mt copy has confounded the cp phylogeny of monotropoids at this locus. The cp-only gene-tree shows perfect agreement with nuclear ITS data.

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1 - The Ohio State University, Museum Of Biological Diversity, 1315 Kinnear Rd, Columbus, OH, 43212, USA
2 - Ohio State University, MUSEUM OF BIOLOGICAL DIVERSITY, 1315 KINNEAR RD, COLUMBUS, OH, 43212-1157, USA


Presentation Type: Oral Paper:Papers for Topics
Session: 35
Location: Pines South/Boise Centre
Date: Wednesday, July 30th, 2014
Time: 11:00 AM
Number: 35012
Abstract ID:691
Candidate for Awards:None

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