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Abstract Detail


Coy , Kiera A [1], Weeks, Andrea [2], Gostel, Morgan [2].

Developing and evaluating novel nuclear markers for phylogenetic reconstruction in the myrrh genus, Commiphora Jacq. (Burseraceae) using multiplexed target enrichment.

To date, widely available nuclear genetic markers (e.g., ETS, ITS) have not provided sufficient variation to fully resolve the evolutionary relationships among the ca. 200 species of Commiphora. Here we use freely available genomic resources to expand the range of genetic markers that can be used for comparative phylogenetic analyses and are optimized for parallel enrichment and sequencing. This poster describes the development and evaluation of these markers from a target pool of about 950 putative shared single-copy nuclear genes using Madagascan species of Commiphora to test them. These closely related species have radiated relatively recently and are a good model for selecting the most informative markers across the genome. Development of these markers followed a bioinformatics pipeline we developed that compares a draft transcriptome of Bursera simaruba against a pool of putative single-copy, orthologous nuclear genes shared across angiosperms. Our analysis identified a subset of approximately 240 genes that aligned to the Bursera simaruba transcriptome and our target gene pool. We demonstrate the effectiveness of a subset of markers for comparative phylogenetic analyses and propose criteria for future research that will consist of primer optimization, size standardization of target products, and sequencing the targets using microfluidic PCR and multiplexed next-generation sequencing. Sequences from these new markers will allow us to better understand the rapid diversification of Commiphora in Madagascar. These gene regions may be useful for phylogenetic analysis in resolving challenging species-level relationships in other angiosperm groups beyond Commiphora and Burseraceae. We will develop test these markers more broadly in other angiosperm lineages for comparative phylogenetics at broad and shallow-scales.

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1 - George Mason University, Department of Biology, 4400 University Dr, Exploratory Hall Room 1201, Fairfax, VA, 22033, USA
2 - George Mason University, Environmental Science And Policy, David King Hall, MSN 5F2, 4400 University Dr., Fairfax, VA, 22030-4444, USA

none specified

Presentation Type: Poster:Posters for Topics
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PPL005
Abstract ID:756
Candidate for Awards:None

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